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High activity CD40L construct : MegaCD40L
 
来源: zkf_ly 加入时间: 2007-01-30 10:33:09 浏览次数:3567
MegaCD40L
High activity construct for B-cell activation experiments
 
MegaCD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin. This construct very effectively simulates the natural membrane-assisted aggreggation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L + enhancer] combinations.
Please see (image001.jpg)
Graphic: MegaCD40L - two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin
Please see (image002.jpg)
Figure: MegaCD40L, Soluble (human) (rec.) (Prod. No. ALX-522-110) does not need an enhancer to induce B cell activation.
Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100祃 RPMI supplemented with 10% FCS) for 24 hours at 37癈 with the indicated concentration of MegaCD40L, Soluble (human) (rec.) or CD40L; Soluble (human) (rec.) (Prod. No. ALX-522-015) in the presence and absence of 1礸/ml enhancer (Prod. No. ALX-804-034). Cells were washed with PBS and stained with 2 祃 each CD86-PE and CD19-FITC in 50 祃 FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 min. at 4癈 in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
Lit. Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)
Ordering information and further details:
ALX-522-110-C010   MegaCD40L 
Related products:
CD40 and CD40L products from ALEXIS
Other useful Mega-Ligands from ALEXIS
 

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